A Novel Breakthrough Assay For Enhanced Selection Of Interfacial Enzymes

One of the principle challenges towards understanding and using enzymes operating at interfaces is that substrate mimetics do not fully capture the complex physical and chemical properties of the intended substrate surface. This work focuses on developing novel generalisable assays for analysing the activities of plastic degrading enzymes directly at plastic surfaces. With applications in laboratory evolution, these assays will allow us to develop the next generations of plastic degrading enzymes forming a critical cornerstone of the circular plastic economy.