Genetic Proteomic Analysis of he P2X7 Purinoceptor in Health and Disease - An international Collaboration between UoP and MIHE Poland

P2X7 purinoceptor has a key role in both initiation and resolution of tissue trauma and inflammation. However, its organization and function are not understood sufficiently to develop targeted treatments. Our laboratory has created a unique P2X7 knock-in/knock-out mouse expressing tagged P2X7 receptors and with loxP sites flanking P2X7 gene in situ. This model takes functional studies to an unprecedented level, allowing genetic-proteomic analyses and specific Cre recombinase ablation. This project aim is to use molecular, proteomic, functional and advanced microscopy methods to identify P2X7 assemblies and interacting proteins, cellular and sub-cellular localization and functional roles in specific cells during tissue injury.