3-Penten-2-one, a novel aldehyde adduct, is a biomarker for increased acetaldehyde in urine

V. Walker; Graham Mills; E. Stansbridge

doi:10.1016/j.jchromb.2009.02.017

3-Penten-2-one, a novel aldehyde adduct, is a biomarker for increased acetaldehyde in urine

V. Walker, Graham Mills, E. Stansbridge

School of Pharmacy & Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Diagnostic profiling of urine for volatile compounds of around 400 patients using headspace solid-phase microextraction (HS-SPME) in alkaline conditions identified 3-penten-2-one (approximately 1 to >6.3 micromol/L) in 26 patients. Five were in barbiturate coma. 3-Penten-2-one, previously of unknown origin, was shown to be formed by aldol condensation of acetaldehyde with acetone or acetoacetate during analysis. Semi-quantification of acetaldehyde using in-fibre derivatisation HS-SPME, showed high concentrations in five urine (33-348 micromol/L) and two plasma (17 and 43 micromol/L) samples. Hence, urinary 3-penten-2-one is a useful biomarker for increased accumulation of acetaldehyde during abnormal metabolic stress.

Original language	English
Pages (from-to)	784-790
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	877
Issue number	8-9
DOIs	https://doi.org/10.1016/j.jchromb.2009.02.017
Publication status	Published - 2009

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title = "3-Penten-2-one, a novel aldehyde adduct, is a biomarker for increased acetaldehyde in urine",

abstract = "Diagnostic profiling of urine for volatile compounds of around 400 patients using headspace solid-phase microextraction (HS-SPME) in alkaline conditions identified 3-penten-2-one (approximately 1 to >6.3 micromol/L) in 26 patients. Five were in barbiturate coma. 3-Penten-2-one, previously of unknown origin, was shown to be formed by aldol condensation of acetaldehyde with acetone or acetoacetate during analysis. Semi-quantification of acetaldehyde using in-fibre derivatisation HS-SPME, showed high concentrations in five urine (33-348 micromol/L) and two plasma (17 and 43 micromol/L) samples. Hence, urinary 3-penten-2-one is a useful biomarker for increased accumulation of acetaldehyde during abnormal metabolic stress.",

author = "V. Walker and Graham Mills and E. Stansbridge",

year = "2009",

doi = "10.1016/j.jchromb.2009.02.017",

language = "English",

volume = "877",

pages = "784--790",

journal = "Journal of Chromatography B: Biomedical Sciences and Applications",

issn = "1387-2273",

publisher = "Elsevier BV",

number = "8-9",

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T1 - 3-Penten-2-one, a novel aldehyde adduct, is a biomarker for increased acetaldehyde in urine

AU - Walker, V.

AU - Mills, Graham

AU - Stansbridge, E.

PY - 2009

Y1 - 2009

N2 - Diagnostic profiling of urine for volatile compounds of around 400 patients using headspace solid-phase microextraction (HS-SPME) in alkaline conditions identified 3-penten-2-one (approximately 1 to >6.3 micromol/L) in 26 patients. Five were in barbiturate coma. 3-Penten-2-one, previously of unknown origin, was shown to be formed by aldol condensation of acetaldehyde with acetone or acetoacetate during analysis. Semi-quantification of acetaldehyde using in-fibre derivatisation HS-SPME, showed high concentrations in five urine (33-348 micromol/L) and two plasma (17 and 43 micromol/L) samples. Hence, urinary 3-penten-2-one is a useful biomarker for increased accumulation of acetaldehyde during abnormal metabolic stress.

AB - Diagnostic profiling of urine for volatile compounds of around 400 patients using headspace solid-phase microextraction (HS-SPME) in alkaline conditions identified 3-penten-2-one (approximately 1 to >6.3 micromol/L) in 26 patients. Five were in barbiturate coma. 3-Penten-2-one, previously of unknown origin, was shown to be formed by aldol condensation of acetaldehyde with acetone or acetoacetate during analysis. Semi-quantification of acetaldehyde using in-fibre derivatisation HS-SPME, showed high concentrations in five urine (33-348 micromol/L) and two plasma (17 and 43 micromol/L) samples. Hence, urinary 3-penten-2-one is a useful biomarker for increased accumulation of acetaldehyde during abnormal metabolic stress.

U2 - 10.1016/j.jchromb.2009.02.017

DO - 10.1016/j.jchromb.2009.02.017

M3 - Article

VL - 877

SP - 784

EP - 790

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 1387-2273

IS - 8-9

ER -

3-Penten-2-one, a novel aldehyde adduct, is a biomarker for increased acetaldehyde in urine

Abstract

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