A fluorescence polarization assay for inhibitors of Hsp90

R. Howes, Xavier Barril, Brian Dymock, Katherine Grant, C. J. Northfield, A. G. S. Robertson, Allan Surgenor, J. Wayne, Lisa Wright, Karen Ball, Thomas P. Matthews, K.-M. Cheung, E. MacDonald, Paul Workman, Martin J. Drysdale

Research output: Contribution to journalArticlepeer-review

Abstract

Hsp90 encodes a ubiquitous molecular chaperone protein conserved among species which acts on multiple substrates, many of which are important cell-signaling proteins. Inhibition of Hsp90 function has been promoted as a mechanism to degrade client proteins involved in tumorigenesis and disease progression. Several assays to monitor inhibition of Hsp90 function currently exist but are limited in their use for a drug discovery campaign. Using data from the crystal structure of an initial hit compound, we have developed a Xuorescence polarization assay to monitor binding of compounds to the ATP-binding site of Hsp90. This assay is very robust (Z' > 0.9) and can detect aYnity of compounds with IC50s to 40 nM. We have used this assay in conjunction with cocrystal structures of small molecules to drive a structure-based design program aimed at the discovery and optimization of a novel class of potent Hsp90 inhibitors.
Original languageEnglish
Pages (from-to)202-213
JournalAnalytical Biochemistry
Volume350
Issue number2
Early online date23 Jan 2006
DOIs
Publication statusPublished - 15 Mar 2006

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