A nuclear phylogenomic study of the angiosperm order Myrtales, exploring the potential and limitations of the universal Angiosperms353 probe set

Olivier Maurin*, Artemis Anest, Sidonie Bellot, Edward Biffin, Grace Brewer, Tristan Charles-Dominique, Robyn S. Cowan, Steven Dodsworth, Niroshini Epitawalage, Berta Gallego, Augusto Giaretta, Renato Goldenberg, Deise J. P. Gonçalves, Shirley Graham, Peter Hoch, Fiorella Mazine, Yee Wen Low, Catherine McGinnie, Fabián A. Michelangeli, Sarah MorrisDarin S. Penneys, Oscar Alejandro Pérez Escobar, Yohan Pillon, Lisa Pokorny, Gustavo Shimizu, Vanessa G. Staggemeier, Andrew H. Thornhill, Kyle W. Tomlinson, Ian M. Turner, Thais Vasconcelos, Peter G. Wilson, Alexandre R. Zuntini, William J. Baker, Félix Forest, Eve Lucas

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

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    Abstract

    Premise: To further advance the understanding of the species-rich, economically and ecologically important angiosperm order Myrtales in the rosid clade, comprising nine families, approximately 400 genera and almost 14,000 species occurring on all continents (except Antarctica), we tested the Angiosperms353 probe kit. 

    Methods: We combined high-throughput sequencing and target enrichment with the Angiosperms353 probe kit to evaluate a sample of 485 species across 305 genera (76% of all genera in the order). 

    Results: Results provide the most comprehensive phylogenetic hypothesis for the order to date. Relationships at all ranks, such as the relationship of the early-diverging families, often reflect previous studies, but gene conflict is evident, and relationships previously found to be uncertain often remain so. Technical considerations for processing HTS data are also discussed. 

    Conclusions: High-throughput sequencing and the Angiosperms353 probe kit are powerful tools for phylogenomic analysis, but better understanding of the genetic data available is required to identify genes and gene trees that account for likely incomplete lineage sorting and/or hybridization events.

    Original languageEnglish
    Pages (from-to)1087-1111
    Number of pages25
    JournalAmerican Journal of Botany
    Volume108
    Issue number7
    Early online date23 Jul 2021
    DOIs
    Publication statusPublished - 27 Jul 2021

    Keywords

    • Alzateaceae
    • Combretaceae
    • Crypteroniaceae
    • Lythraceae
    • Melastomataceae
    • Myrtaceae
    • Onagraceae
    • Penaeaceae
    • phylogenomics
    • Vochysiaceae

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