@inbook{27b95dfcdd48488aa84652638e3655c3,
title = "A Sequence- and Ligation-Independent Cloning (SLIC) procedure for the insertion of genes into a plasmid vector",
abstract = "Molecular cloning is a routine technique for many laboratories with applications from genetic engineering to recombinant protein expression. While restriction-ligation cloning can be slow and inefficient, ligation-independent cloning uses long single-stranded overhangs generated by T4 DNA polymerase{\textquoteright}s 3′ exonuclease activity to anneal the insert and plasmid vector prior to transformation. This chapter describes a fast, high-efficiency protocol for inserting one or more genes into a vector using sequence- and ligation-independent cloning (SLIC).",
keywords = "SLIC, PCR, cloning, T4 polymerase, genetic engineering, protein expression",
author = "Holland, {Robert A.}",
year = "2023",
month = mar,
day = "1",
doi = "10.1007/978-1-0716-3004-4_2",
language = "English",
isbn = "9781071630037",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "25--32",
editor = "Garry Scarlett",
booktitle = "DNA Manipulation and Analysis",
address = "United States",
edition = "1st",
}