Acidithiobacillus sulfuriphilus sp. Nov. An extremely acidophilic sulfur-oxidizing chemolithotroph isolated from a neutral pH environment

Carmen Falagan*, Ana Moya-Beltran, Matías Castro, Raquel Quatrini, D. Barrie Johnson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The genus Acidithiobacillus currently includes seven species with validly published names, which fall into two major groups, those that can oxidize ferrous iron and those that do not. All seven species can use zero-valent sulfur and reduced sulfur oxy-anions as electron donors, are obligately chemolithotrophic and acidophilic bacteria with pH growth optima below 3.0. The 16S rRNA gene of a novel strain (CJ-2T) isolated from circum-neutral pH mine drainage showed 95–97 % relatedness to members of the genus Acidithiobacillus. Digital DNA–DNA hybridization (dDDH) values between strains and whole-genome pairwise comparisons between the CJ-2T strain and the reference genomes available for members of the genus Acidithiobacillus confirmed that CJ-2Trepresents a novel species of this genus. CJ-2T is a strict aerobe, oxidizes zero-valent sulfur and reduced inorganic sulfur compounds but does not use ferrous iron or hydrogen as electron donors. The isolate is mesophilic (optimum growth temperature 25–28C) and extremely acidophilic (optimum growth pH 3.0), though its pH optimum and maximum were significantly higher than those of non-iron-oxidising acidithiobacilli with validly published names. The major fatty acids of CJ-2T were C18: 1!7c, C:16: 1!7c/iso-C15: 0 2-OH, C16: 0 and C19: 0 cyclo !8c and the major respiratory quinone present was Q8. The name Acidithiobacillus sulfuriphilus sp. nov. is proposed, the type strain is CJ2T (=DSM 105150T=KCTC 4683T).

Original languageEnglish
Pages (from-to)2907-2913
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume69
Issue number9
DOIs
Publication statusPublished - 1 Sept 2019

Keywords

  • Acidithiobacillus
  • Acidithiobacillus sulfuriphilus
  • Acidophile
  • PH neutral
  • Sulfur-oxidation
  • UKRI
  • NERC
  • NE/L014076/1

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