Analysis of spatial and temporal gene expression patterns in blastula and gastrula stage chick embryos

Susan C. Chapman, Frank Schubert, G. Schoenwolf, A. Lumsden

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Studies on the genetic basis of rostral–caudal specification, neural induction, and head development require knowledge of the relevant gene expression patterns. Gaps in our understanding of gene expression have led us to examine the detailed spatiotemporal expression patterns of 19 genes implicated in early development, to learn more about their potential role in specifying and patterning early developmental processes leading to head formation. Here, we report the expression patterns of these markers in blastula- and gastrula-stage chick embryos, using whole-mount in situ hybridisation. Nodal, Fgf8, Bmp7, Chordin, Lim1, Hnf3β, Otx2, Goosecoid, Cerberus, Hex, Dickkopf1, and Crescent are all already expressed by the time the egg is laid. When the primitive streak has reached its full length, a later group of genes, including Ganf, Six3, Bmp2, Bmp4, Noggin, Follistatin, and Qin (BF1), begins to be expressed. We reassess current models of early rostral patterning based on the analysis of these dynamic spatiotemporal expression patterns.
    Original languageEnglish
    Pages (from-to)187-199
    Number of pages13
    JournalDevelopmental Biology
    Volume245
    Issue number1
    DOIs
    Publication statusPublished - 2002

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