Animal standardisation for mixed species ecotoxicological studies: Establishing a laboratory breeding programme for Gammarus pulex and Asellus aquaticus

Michelle Bloor

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    Abstract

    This paper outlines how to establish a standardised laboratory breeding programme for Gammarus pulex and Asellus aquaticus. Wild Gammarus pulex and Asellus aquaticus specimens were captured from an unpolluted river source and used as founder populations for the programme. The Gammarus pulex and Asellus aquaticus founder populations were permitted to breed randomly and the subsequent offspring (F1, F2 and F3 generations etc.) were available as standardised test animals for mixed species aquatic toxicity tests. The husbandry required to maintain laboratory breeding populations of Gammarus pulex and Asellus aquaticus is outlined and the animals’ development cycles are discussed.
    Original languageEnglish
    Pages (from-to)179-190
    Number of pages12
    JournalZoologica Baetica
    Volume21
    Publication statusPublished - 2010

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