The development of programmable regulators that precisely and predictably control gene expression is a major goal of synthetic biology. Consequently, rapid high-throughput biochemical methods capable of quantitatively analyzing all components of gene expression would be of value in the characterization and optimization of regulator performance. In this study we demonstrate a novel application of RNA arrays, involving the production of reporter-protein arrays, to gene expression analysis. This method enables simultaneous quantification of both the transcription and post-transcription/translation components of gene expression and it also allows the assessment of the orthogonality of multiple regulators. We use our method to directly compare the performance of a series of previously characterized synthetic post-transcriptional riboregulators, thus demonstrating its utility in the development of synthetic regulatory modules and evaluation of gene expression regulation in general.
|Journal||ACS Synthetic Biology|
|Early online date||25 Jan 2019|
|Publication status||Early online - 25 Jan 2019|
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Supplementary information for 'Application of mRNA arrays for the production of mCherry reporter-protein arrays for quantitative gene expression analysis'.
Norouzi, M. (Creator), Pickford, A. (Creator), Butt, L. (Creator), Vincent, H. (Creator) & Callaghan, A. (Creator), ACS Publications, 25 Jan 2019