@inbook{7f91c9d39eb3426eb0a165faa5773478,
title = "Assembling multiple fragments: the Gibson Assembly",
abstract = "The Gibson Assembly is a popular method for molecular cloning which has been developed specifically to join several fragments together in a specific order, without the constraint of restriction enzyme sites. This method is based on the assembly of overlapping fragments, generally produced by PCR, and then combining them using three enzymes: a 5′ exonuclease, a DNA polymerase, and a DNA ligase, in an isothermal reaction. Here, we describe this method, including the design of primers for the generation of the overlapping fragments and the assembly; to this end, we provide an example involving joining two fragments in a single plasmid.",
keywords = "Gibson Assembly, cloning, overlapping primers",
author = "Luisana Avilan",
year = "2023",
month = mar,
day = "1",
doi = "10.1007/978-1-0716-3004-4_4",
language = "English",
isbn = "9781071630037",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "45--53",
editor = "Garry Scarlett",
booktitle = "DNA Manipulation and Analysis",
address = "United States",
edition = "1st",
}