Organic δ13C and C/N analyses of estuarine deposits provide proxies for changes in the source of organic matter, which can be driven by fluctuations in relative sealevel, river discharge, and catchment disturbance. Here we present the results of a comprehensive vegetation and sediment δ13C and C/N survey of Welwich Marsh (outer HumberEstuary, UK), together with high-resolution δ13C and C/N analyses of Holocenecores collected nine years previously from the HumberEstuary and the Lincolnshire Marshes, Eastern England, UK. The contemporary intertidal δ13C and C/N dataset shows a gradual increase in surface sediment δ13C with decreasing marsh height and suggests that δ13C is controlled by the degree of tidal inundation and thus reflects organic matter source. However, sediment C/Nratios are less sensitive to tidal changes and the recent introduction of C4 salt-marsh species complicates the contemporary analogue. The Holocene δ13C and C/N records are in general agreement with existing microfossil data and provide additional palaeoenvironmental information. This includes support for an estuary-wide expansion of marine conditions from c. 3.3 ka cal. yr BP, followed by a contraction of marine conditions after c. 2.7 ka cal. yr BP, and evidence for an increase in delivery of terrigenous organic matter to the inner estuary in the late Holocene. Bulk organic δ13C and C/N analysis is shown to be a reliable and independent indicator of coastal environmental change and is therefore a complementary technique to the more commonly used microfossil approach. However, this study also shows that in some circumstances the technique may be compromised when applied to sediments from cores that have been stored for a period of time.