Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium

K. S. M. Rahman*, G. Street, R. Lord, G. Kane, I. M. Banat

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A study was undertaken to find methods for enhancing rates of hydrocarbon biodegradation in gasoline-contaminated soil by ex-situ bioremediation. Garden soil was treated with gasoline-spilled soil from a gasoline station and different combinations of amendments were prepared using mixed bacterial consortium, poultry litter, coir pith and rhamnolipid biosurfactant produced by Pseudomonas aeruginosa. Bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB (including seed germination, chlorophyll content, shoot and root length) were measured for a period of 90 days. Approximately 78% of the hydrocarbons were effectively degraded within 60 days in soil samples amended with all additives. Maximum germination rate, shoot length, root length and chlorophyll content in Phaseolus aureus were each recorded after 60 days. Further incubation to 90 days did not cause significant improvements. Statistical analysis using Analysis of Variance and Duncan's Multiple Range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content (1% probability level). All tested additives, including rhamnolipid biosurfactant, had significant positive effects on the bioremediation of gasoline-contaminated soils.

Original languageEnglish
Title of host publicationWIT Transactions on Ecology and the Environment
Pages401-407
Number of pages7
Volume10
Publication statusPublished - 11 Oct 2004
EventFifth International Conference on Environmental Problems in Coastal Regions Incorporating Oil Spill Studies - Alicante, Spain
Duration: 26 Apr 200430 Apr 2004

Publication series

NameEnvironmental Studies
PublisherWIT Press
ISSN (Print)1462-6098

Conference

ConferenceFifth International Conference on Environmental Problems in Coastal Regions Incorporating Oil Spill Studies
Abbreviated titleCOASTAL ENVIRONMENT V
CountrySpain
CityAlicante
Period26/04/0430/04/04

Keywords

  • Amendments
  • Bioremediation
  • Biosurfactant
  • Coir pith
  • Gasoline spilled soil
  • Mixed bacterial consortium
  • Phaseolus aureus RoxB
  • Poultry litter

Fingerprint

Dive into the research topics of 'Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium'. Together they form a unique fingerprint.

Cite this