Carbohydrate active enzymes applied in the production of functional oligosaccharides

Fabiano Jares Contesini, Evandro A. de Lima, Fernanda Mandelli, Gustavo Borin, Rafael F. Alves, César Rafael F. Terrasan

Research output: Chapter in Book/Report/Conference proceedingEntry for encyclopedia/dictionary


Functional foods are receiving major attention during the last decades due to their health promoting effects, including stimulation of immune system, and anticancerogenic properties. One highlighted group of bioactive compounds corresponds to prebiotics, consisting of non-digestible oligosaccharides that stimulate selective growth and activity of benefic bacteria in human colon. One of the most interesting form of functional oligosaccharides production is using carbohydrate active enzymes, mainly glycoside hydrolases with hydrolysis and transglycosylation activity. The use of microbial enzymes can be advantageous when compared to natural extraction, or chemical synthesis, rendering higher process yield and lower production of undesirable byproducts. Examples of prebiotics are fructo-, galacto- and xylooligosaccharides which are efficiently produced by hydrolases with fructosyltransferase, galactosyltransferases and xylanase activity, respectively. In addition, several other oligosaccharides can be produced using different enzymes.
Original languageEnglish
Title of host publicationEncyclopedia of Food Chemistry
EditorsLaurence Melton, Fereidoon Shahidi, Peter Varelis
Number of pages5
ISBN (Print)9780128140451
Publication statusPublished - 30 Nov 2018


  • functional foods
  • CAZymes
  • fructooligosaccharides
  • galactooligosaccharides
  • xylooligosaccharides
  • prebiotics
  • dietic fibers
  • glycoside hydrolases
  • transglycosylation activity
  • fructosyltransferases
  • xylosidases
  • galactosyltransferase

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