Circular dichroism for the analysis of protein–DNA interactions

Giuliano Siligardi, George Geoffrey Kneale, Garry Scarlett

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

The aim of this chapter is to provide information on the practical aspects of circular dichroism (CD) and synchrotron radiation circular dichroism (SRCD) in protein–nucleic acids interaction solution studies. The chapter will describe the guidelines appropriate to designing experiments and conducting correct data interpretation, the use of both benchtop and synchrotron CD approaches is discussed and the advantages of SRCD outlined. Further information and a good general review of the field a can be found in Gray (Circular Dichroism of protein–nucleic acid interactions. In: Fasman GD (ed) Circular dichroism and the conformational analysis of biomolecules. Plenum Press, New York. pp 469–500, 1996).
Original languageEnglish
Title of host publicationDNA-protein interactions
Subtitle of host publicationprinciples and protocols
EditorsBenoît P. Leblanc, Sebastien Rodrigue
Place of PublicationCham
PublisherHumana Press
Pages299-312
Edition4
ISBN (Electronic)9781493928774
ISBN (Print)9781493928767
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume1334
ISSN (Electronic)1064-3745

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