Purpose The purpose of this study was to evaluate, using a clinical spectrophotometer, the ability to color match shades selected with the 3D-Master shade guide using 2 different ceramic systems. Material and methods Two ceramic systems were selected for this study, the VITA Omega 900 and VITA VM 13. For both systems, shades 2M3, 3M2, 4M2 of the VITA 3D-Master shade guide were selected. Thirty ceramic discs, 15 mm in diameter, were fabricated with different layer thickness ratios of opaque dentin, dentin, and enamel, maintaining a constant overall thickness. A clinical spectrophotometer (VITA Easyshade) was used for color comparison. The measured ΔE values were statistically analyzed with a 3-way ANOVA and the Tukey HSD test (α=.05). Results The 3-way ANOVA showed that the ceramic system (P<.001), the selected shade (P<.001), and the layering scheme all significantly influenced the ΔE values (P<.001). Conclusions Both VITA VM 13 and VITA Omega 900 ceramic systems showed a color match with the shades 2M3, 3M2, and 4M2 of the VITA 3D-Master shade guide to be within the limits for clinical acceptability (ΔE≤ 3.3). VM 13 showed a statistically better color match. The layering scheme influenced the definitive color of the restoration.