TY - JOUR
T1 - Combination of Sanger and target-enrichment markers supports revised generic delimitation in the problematic ‘Urera clade’ of the nettle family (Urticaceae)
AU - Wells, Tom
AU - Maurin, Olivier
AU - Dodsworth, Steven
AU - Friis, Ib
AU - Cowan, Robyn
AU - Epitawalage, Niroshini
AU - Brewer, Grace
AU - Forest, Felix
AU - Baker, William J.
AU - Monro, Alexandre K.
N1 - Funding Information:
Main Funding provided by The Royal Botanic Garden Edinburgh, as part of MSc programme, with High-Throughput Sequencing undertaken as part of the Plant and Fungal Tree of Life project funded by the Calleva Foundation and the Sackler Trust.
Funding Information:
The authors would like to thank Andrew Hudson (University of Edinburgh) for help with SANGER sequencing; Robyn Cowan in the Jodrell laboratory for the targeted sequencing; David Harris for his observations on Urera in Central Africa; Nicholas Hind for his advice on nomenclature; Juliet Beentje for Fig. 5 and the Curators of Herbaria at BM, E, and K for access to and sampling of collections. Funding for the Targeted Sequencing was provided as part of the PAFTOL project by The Sackler Trust and Calleva Foundation.
Funding Information:
The authors would like to thank Andrew Hudson (University of Edinburgh) for help with SANGER sequencing; Robyn Cowan in the Jodrell laboratory for the targeted sequencing; David Harris for his observations on Urera in Central Africa; Nicholas Hind for his advice on nomenclature; Juliet Beentje for Fig. 5 and the Curators of Herbaria at BM, E, and K for access to and sampling of collections. Funding for the Targeted Sequencing was provided as part of the PAFTOL project by The Sackler Trust and Calleva Foundation. Main Funding provided by The Royal Botanic Garden Edinburgh, as part of MSc programme, with High-Throughput Sequencing undertaken as part of the Plant and Fungal Tree of Life project funded by the Calleva Foundation and the Sackler Trust.
Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Urera Gaudich, s.l. is a pantropical genus comprising c. 35 species of trees, shrubs, and vines. It has a long history of taxonomic uncertainty, and is repeatedly recovered as polyphyletic within a poorly resolved complex of genera in the Urticeae tribe of the nettle family (Urticaceae). To provide generic delimitations concordant with evolutionary history, we use increased taxonomic and genomic sampling to investigate phylogenetic relationships among Urera and associated genera. A cost-effective two-tier genome-sampling approach provides good phylogenetic resolution by using (i) a taxon-dense sample of Sanger sequence data from two barcoding regions to recover clades of putative generic rank, and (ii) a genome-dense sample of target-enrichment data for a subset of representative species from each well-supported clade to resolve relationships among them. The results confirm the polyphyly of Urera s.l. with respect to the morphologically distinct genera Obetia, Poikilospermum and Touchardia. Afrotropic members of Urera s.l. are recovered in a clade sister to the xerophytic African shrubs Obetia; and Hawaiian ones with Touchardia, also from Hawaii. Combined with distinctive morphological differences between Neotropical and African members of Urera s.l., these results lead us to resurrect the previously synonymised name Scepocarpus Wedd. for the latter. The new species epiphet Touchardia oahuensis T.Wells & A.K. Monro is offered as a replacement name for Touchardia glabra non H.St.John, and subgenera are created within Urera s.s. to account for the two morphologically distinct Neotropical clades. This new classification minimises taxonomic and nomenclatural disruption, while more accurately reflecting evolutionary relationships within the group.
AB - Urera Gaudich, s.l. is a pantropical genus comprising c. 35 species of trees, shrubs, and vines. It has a long history of taxonomic uncertainty, and is repeatedly recovered as polyphyletic within a poorly resolved complex of genera in the Urticeae tribe of the nettle family (Urticaceae). To provide generic delimitations concordant with evolutionary history, we use increased taxonomic and genomic sampling to investigate phylogenetic relationships among Urera and associated genera. A cost-effective two-tier genome-sampling approach provides good phylogenetic resolution by using (i) a taxon-dense sample of Sanger sequence data from two barcoding regions to recover clades of putative generic rank, and (ii) a genome-dense sample of target-enrichment data for a subset of representative species from each well-supported clade to resolve relationships among them. The results confirm the polyphyly of Urera s.l. with respect to the morphologically distinct genera Obetia, Poikilospermum and Touchardia. Afrotropic members of Urera s.l. are recovered in a clade sister to the xerophytic African shrubs Obetia; and Hawaiian ones with Touchardia, also from Hawaii. Combined with distinctive morphological differences between Neotropical and African members of Urera s.l., these results lead us to resurrect the previously synonymised name Scepocarpus Wedd. for the latter. The new species epiphet Touchardia oahuensis T.Wells & A.K. Monro is offered as a replacement name for Touchardia glabra non H.St.John, and subgenera are created within Urera s.s. to account for the two morphologically distinct Neotropical clades. This new classification minimises taxonomic and nomenclatural disruption, while more accurately reflecting evolutionary relationships within the group.
KW - Angiosperms353
KW - generic delimitation
KW - Lianas
KW - secondary woodiness
UR - http://www.scopus.com/inward/record.url?scp=85101020506&partnerID=8YFLogxK
UR - https://uobrep.openrepository.com/handle/10547/624881
U2 - 10.1016/j.ympev.2020.107008
DO - 10.1016/j.ympev.2020.107008
M3 - Article
C2 - 33160040
AN - SCOPUS:85101020506
SN - 1055-7903
VL - 158
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
M1 - 107008
ER -