Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces

Elena García-Gareta, Jia Hua, Jonathan C Knowles, Gordon W Blunn

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods-biomimetic (BioM), electrochemical at 20 mA/cm(2) and at 6.5 mA/cm(2)-and cultured with MSCs. At days 4, 7 and 14, cell proliferation-alamarBlue(®) activity and DNA quantification-and differentiation down the osteogenic lineage-ALP activity normalised per amount of DNA and SEM (morphology)-were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.

    Original languageEnglish
    Pages (from-to)199-210
    Number of pages12
    JournalJournal of Materials Science: Materials in Medicine
    Volume24
    Issue number1
    DOIs
    Publication statusPublished - Jan 2013

    Keywords

    • Alkaline Phosphatase
    • Animals
    • Biomimetics
    • Calcium Phosphates
    • Cell Differentiation
    • Cell Proliferation
    • Coated Materials, Biocompatible
    • DNA
    • Electrochemical Techniques
    • Mesenchymal Stromal Cells
    • Microscopy, Electron, Scanning
    • Sheep
    • Comparative Study
    • Research Support, Non-U.S. Gov't

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