Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces

Elena García-Gareta, Jia Hua, Jonathan C Knowles, Gordon W Blunn

Research output: Contribution to journalArticlepeer-review

Abstract

The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods-biomimetic (BioM), electrochemical at 20 mA/cm(2) and at 6.5 mA/cm(2)-and cultured with MSCs. At days 4, 7 and 14, cell proliferation-alamarBlue(®) activity and DNA quantification-and differentiation down the osteogenic lineage-ALP activity normalised per amount of DNA and SEM (morphology)-were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.

Original languageEnglish
Pages (from-to)199-210
Number of pages12
JournalJournal of Materials Science: Materials in Medicine
Volume24
Issue number1
DOIs
Publication statusPublished - Jan 2013

Keywords

  • Alkaline Phosphatase
  • Animals
  • Biomimetics
  • Calcium Phosphates
  • Cell Differentiation
  • Cell Proliferation
  • Coated Materials, Biocompatible
  • DNA
  • Electrochemical Techniques
  • Mesenchymal Stromal Cells
  • Microscopy, Electron, Scanning
  • Sheep
  • Comparative Study
  • Research Support, Non-U.S. Gov't

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