Projects per year
Enzyme-based depolymerization is a viable approach for recycling of poly(ethylene terephthalate) (PET). PETase from Ideonella sakaiensis (IsPETase) is capable of PET hydrolysis under mild conditions but suffers from concentration-dependent inhibition. Here, we report that this inhibition is dependent on incubation time, the solution conditions and PET surface area. Furthermore, this inhibition is evident in other mesophilic PET-degrading enzymes to varying degrees, independent of the level of PET depolymerization activity. The inhibition has no clear structural basis, but moderately thermostable IsPETase variants exhibit reduced inhibition, and the property is completely absent in the highly thermostable HotPETase, previously engineered by directed evolution, which our simulations suggest results from reduced flexibility around the active site. This work highlights a limitation in applying natural mesophilic hydrolases for PET hydrolysis, and reveals an unexpected positive outcome of engineering these enzymes for enhanced thermostability.
|Early online date||21 Feb 2023|
|Publication status||Early online - 21 Feb 2023|
- mesophilic enzymes
- interfacial biocatalysis
- PET hydrolase
CEI: Centre for Enzymes Innovation (CEI) Expansion
Pickford, A., Duckering, S., Miles, R., Lichtenstein, B. & McGeehan, J.
12/11/20 → …
New enzymes for degrading natural and synthetic polymers: A combined structural and synthetic biology approach
Pickford, A. & McGeehan, J.
1/10/18 → 30/09/23
Centre for Enzyme Innovation
McGeehan, J., Callaghan, A., Cragg, S., Pickford, A., Watts, J., Rahman, P., Cox, P. & Robson, S.
1/08/19 → 31/07/22