@misc{80838c9fd3ec429ba288e2d09825fc0e,
title = "Confirming antibody specificity in Xenopus",
abstract = "Verifying that a new antibody recognizes its target can be difficult. In this protocol, expression of a target protein in Xenopus embryos is either knocked down using CRISPR–Cas9 technology (for zygotic proteins) or enhanced by microinjection of a synthetic mRNA (for maternal proteins). Western blotting analysis is then performed. If the antibody recognizes the target protein, the western blot will show a relatively weak band for CRISPR-injected embryos and a relatively strong band for RNA-injected embryos. This represents a straightforward, powerful strategy for confirming antibody specificity in Xenopus.",
author = "Martin, {Sian A.} and Page, {Suzannah J.} and Maya Piccinni and Matt Guille",
year = "2020",
month = dec,
day = "1",
doi = "10.1101/pdb.prot105601",
language = "English",
volume = "2020",
series = "Cold Spring Harbor Protocols",
publisher = "Cold Spring Harbor Laboratory Press",
address = "United States",
edition = "12",
type = "Other",
}