Control of a multisubunit DNA motor by a thermoresponsive polymer switch

S. Pennadam, M. Lavigne, C. Dutta, K. Firman, Darren Mernagh, Darek Gorecki, C. Alexander

Research output: Contribution to journalArticlepeer-review

Abstract

The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction−modification (R−M) enzymes enables temperature-controlled “switching” of DNA methylation by the conjugate. Polymers attached to the enzyme at a subunit distal to the methylation subunit allow retention of DNA recognition and ATPase activity while controlling methylation of plasmid DNA. This regulation of enzyme activity arises from the coil−globule phase transitions of the polymer as shown in light scattering and gel retardation assays.
Original languageEnglish
Pages (from-to)13208-13209
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number41
DOIs
Publication statusPublished - 2004

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