Control of a multisubunit DNA motor by a thermoresponsive polymer switch

S. Pennadam; M. Lavigne; C. Dutta; K. Firman; Darren Mernagh; Darek Gorecki; C. Alexander

doi:10.1021/ja045275j

Control of a multisubunit DNA motor by a thermoresponsive polymer switch

S. Pennadam
, M. Lavigne
, C. Dutta
, K. Firman
, Darren Mernagh
, Darek Gorecki
, C. Alexander

Research output: Contribution to journal › Article › peer-review

Abstract

The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction−modification (R−M) enzymes enables temperature-controlled “switching” of DNA methylation by the conjugate. Polymers attached to the enzyme at a subunit distal to the methylation subunit allow retention of DNA recognition and ATPase activity while controlling methylation of plasmid DNA. This regulation of enzyme activity arises from the coil−globule phase transitions of the polymer as shown in light scattering and gel retardation assays.

Original language	English
Pages (from-to)	13208-13209
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	126
Issue number	41
DOIs	https://doi.org/10.1021/ja045275j
Publication status	Published - 2004

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title = "Control of a multisubunit DNA motor by a thermoresponsive polymer switch",

abstract = "The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction−modification (R−M) enzymes enables temperature-controlled “switching” of DNA methylation by the conjugate. Polymers attached to the enzyme at a subunit distal to the methylation subunit allow retention of DNA recognition and ATPase activity while controlling methylation of plasmid DNA. This regulation of enzyme activity arises from the coil−globule phase transitions of the polymer as shown in light scattering and gel retardation assays.",

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AU - Pennadam, S.

AU - Lavigne, M.

AU - Dutta, C.

AU - Firman, K.

AU - Mernagh, Darren

AU - Gorecki, Darek

AU - Alexander, C.

PY - 2004

Y1 - 2004

N2 - The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction−modification (R−M) enzymes enables temperature-controlled “switching” of DNA methylation by the conjugate. Polymers attached to the enzyme at a subunit distal to the methylation subunit allow retention of DNA recognition and ATPase activity while controlling methylation of plasmid DNA. This regulation of enzyme activity arises from the coil−globule phase transitions of the polymer as shown in light scattering and gel retardation assays.

AB - The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction−modification (R−M) enzymes enables temperature-controlled “switching” of DNA methylation by the conjugate. Polymers attached to the enzyme at a subunit distal to the methylation subunit allow retention of DNA recognition and ATPase activity while controlling methylation of plasmid DNA. This regulation of enzyme activity arises from the coil−globule phase transitions of the polymer as shown in light scattering and gel retardation assays.

U2 - 10.1021/ja045275j

DO - 10.1021/ja045275j

M3 - Article

SN - 0002-7863

VL - 126

SP - 13208

EP - 13209

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 41

ER -

Control of a multisubunit DNA motor by a thermoresponsive polymer switch

Abstract

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