Copper plate replica SEM of developing dental plaque in monkeys (Macaca fascicularis)

The aim of the study was to examine the development of dental plaque in macaque monkeys (Macaca fascicularis). Copper plate replicas were constructed from impressions of the labial surface of one of the upper central incisor teeth after tooth cleaning and when plaque had accumulated for 12, 24 and 48 h in each of five animals. Scanning electron microscope examination of the replicas showed that bacteria were present on the tooth surface as scattered individual cells, which after 24 h had formed a continuous layer. Both coccoid and filamentous bacteria were visible in 48-hour-old plaque. Such a rapid accumulation of potentially periodontopathic dental plaque emphasizes the importance of regular and meticulous oral hygiene.