Crystal quality and inhibitor binding by aspartic proteinases; preparation of high quality crystals of mouse renin

M. Badasso; B. l. Sibanda; J. B. Cooper; C. G. Dealwis; S. P. Wood

doi:10.1016/0022-0248(92)90274-M

Crystal quality and inhibitor binding by aspartic proteinases; preparation of high quality crystals of mouse renin

M. Badasso, B. l. Sibanda, J. B. Cooper, C. G. Dealwis, S. P. Wood

Institute of Life Sciences and Healthcare

Research output: Contribution to journal › Article › peer-review

Abstract

Renin from mouse submandibular glands has been highly purified and co-crystallized with a synthetic nonapeptide fragment of rat angiotensionogen in which the scissile Leu-Leu bond has been modified as a hydroxyethylene mimic of the transition state. The strong diffraction from these crystals compared to the native form is discussed in relation to the behaviour of other members of the aspartic proteinase family in crystallisation.

Original language	English
Pages (from-to)	393-399
Journal	Journal of Crystal Growth
Volume	122
Issue number	1-4
DOIs	https://doi.org/10.1016/0022-0248(92)90274-M
Publication status	Published - 1 Aug 1992

Access to Document

10.1016/0022-0248(92)90274-M

http://linkinghub.elsevier.com/retrieve/pii/002202489290274M

Cite this

@article{9d11f70ffdde469c945827021868d117,

title = "Crystal quality and inhibitor binding by aspartic proteinases; preparation of high quality crystals of mouse renin",

abstract = "Renin from mouse submandibular glands has been highly purified and co-crystallized with a synthetic nonapeptide fragment of rat angiotensionogen in which the scissile Leu-Leu bond has been modified as a hydroxyethylene mimic of the transition state. The strong diffraction from these crystals compared to the native form is discussed in relation to the behaviour of other members of the aspartic proteinase family in crystallisation.",

author = "M. Badasso and Sibanda, {B. l.} and Cooper, {J. B.} and Dealwis, {C. G.} and Wood, {S. P.}",

year = "1992",

month = aug,

day = "1",

doi = "10.1016/0022-0248(92)90274-M",

language = "English",

volume = "122",

pages = "393--399",

journal = "Journal of Crystal Growth",

issn = "0022-0248",

publisher = "Elsevier",

number = "1-4",

}

TY - JOUR

T1 - Crystal quality and inhibitor binding by aspartic proteinases; preparation of high quality crystals of mouse renin

AU - Badasso, M.

AU - Sibanda, B. l.

AU - Cooper, J. B.

AU - Dealwis, C. G.

AU - Wood, S. P.

PY - 1992/8/1

Y1 - 1992/8/1

N2 - Renin from mouse submandibular glands has been highly purified and co-crystallized with a synthetic nonapeptide fragment of rat angiotensionogen in which the scissile Leu-Leu bond has been modified as a hydroxyethylene mimic of the transition state. The strong diffraction from these crystals compared to the native form is discussed in relation to the behaviour of other members of the aspartic proteinase family in crystallisation.

AB - Renin from mouse submandibular glands has been highly purified and co-crystallized with a synthetic nonapeptide fragment of rat angiotensionogen in which the scissile Leu-Leu bond has been modified as a hydroxyethylene mimic of the transition state. The strong diffraction from these crystals compared to the native form is discussed in relation to the behaviour of other members of the aspartic proteinase family in crystallisation.

U2 - 10.1016/0022-0248(92)90274-M

DO - 10.1016/0022-0248(92)90274-M

M3 - Article

SN - 0022-0248

VL - 122

SP - 393

EP - 399

JO - Journal of Crystal Growth

JF - Journal of Crystal Growth

IS - 1-4

ER -

Crystal quality and inhibitor binding by aspartic proteinases; preparation of high quality crystals of mouse renin

Abstract

Access to Document

Fingerprint

Cite this