Our results show for the first time that in both chicken and mouse, Dact1 is expressed during myogenesis, with a strong upregulation as cells engage in terminal differentiation, cell cycle withdrawal and cell fusion. In humans, Dact1 expression was found to be altered in specific muscle pathologies, including muscular dystrophies. Our bioinformatic analyses of Dact1 proteins revealed long intrinsically disordered regions, which may underpin the ability of Dact1 to interact with its many partners in the various Wnt pathways. In addition, we found that Dact1 has strong propensity for liquid-liquid phase separation, a feature that explains its ability to form nuclear aggregates and points to a possible role as a molecular ‘on’-‘off’ switch.
Taken together, our data suggest Dact1 as a candidate, multi-faceted regulator of amniote myogenesis with a possible pathophysiological role in human muscular diseases.
|Number of pages||13|
|Journal||Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology|
|Early online date||9 Jul 2021|
|Publication status||Published - 1 Oct 2021|
- skeletal myogenesis
- wnt signaling
- chicken primary myoblast
- C2C12 cells
- intrinsically disordered proteins
- muscular diseases