TY - JOUR
T1 - Development and automation of a test of impulse control in zebrafish
AU - Parker, Matthew O.
AU - Ife, Dennis
AU - Ma, Jun
AU - Pancholi, Mahesh
AU - Smeraldi, Fabrizio
AU - Straw, Chris
AU - Brennan, Caroline H.
PY - 2013/10
Y1 - 2013/10
N2 - Deficits in impulse control (difficulties in inhibition of a pre-potent response) are
fundamental to a number of psychiatric disorders, but the molecular and cellular basis
is poorly understood. Zebrafish offer a very useful model for exploring these mechanisms,
but there is currently a lack of validated procedures for measuring impulsivity in fish. In
mammals, impulsivity can be measured by examining rates of anticipatory responding in
the 5-choice serial reaction time task (5-CSRTT), a continuous performance task where
the subject is reinforced upon accurate detection of a briefly presented light in one of
five distinct spatial locations. This paper describes the development of a fully-integrated
automated system for testing impulsivity in adult zebrafish. We outline the development
of our image analysis software and its integration with National Instruments drivers and
actuators to produce the system. We also describe an initial validation of the system
through a one-generation screen of chemically mutagenized zebrafish, where the testing
parameters were optimized.
AB - Deficits in impulse control (difficulties in inhibition of a pre-potent response) are
fundamental to a number of psychiatric disorders, but the molecular and cellular basis
is poorly understood. Zebrafish offer a very useful model for exploring these mechanisms,
but there is currently a lack of validated procedures for measuring impulsivity in fish. In
mammals, impulsivity can be measured by examining rates of anticipatory responding in
the 5-choice serial reaction time task (5-CSRTT), a continuous performance task where
the subject is reinforced upon accurate detection of a briefly presented light in one of
five distinct spatial locations. This paper describes the development of a fully-integrated
automated system for testing impulsivity in adult zebrafish. We outline the development
of our image analysis software and its integration with National Instruments drivers and
actuators to produce the system. We also describe an initial validation of the system
through a one-generation screen of chemically mutagenized zebrafish, where the testing
parameters were optimized.
U2 - 10.3389/fnsys.2013.00065
DO - 10.3389/fnsys.2013.00065
M3 - Article
SN - 1662-5137
VL - 7
SP - 65
JO - Frontiers in Systems Neuroscience
JF - Frontiers in Systems Neuroscience
ER -