Development of a simple and low cost microbioreactor for high-throughput bioprocessing

Pattanathu K. S. M. Rahman, Godfrey Pasirayi, Vincent Auger, Zulfiqur Ali

Research output: Contribution to journalArticlepeer-review


A simple microbioreactor for high-throughput bioprocessing made from low cost polymer polytetrafluoroethylene (PTFE) tubes with a working volume of 1.5 ml is described. We have developed a microfluidic system that handles a small population of cells of a model microorganism, Pseudomonas aeruginosa DS10-129. Under the conditions of the microbioreactor, the organism produced extracellular secondary metabolites by using nutrient broth modified with glycerol. Pyocyanins were isolated from the fermented medium as a metabolite of interest. Antibiotic properties of pyocyanin were effective against a number of microorganisms such as Staphylococcus aureus, S. epidermis, Bacillus subtilis, Micrococcus luteus and Saccharomyces cerevisiae. Batch fermentation of the model organism in the microbioreactor was compared to shake-flask and conventional bench fermenter methods. Results obtained from the microbioreactor compared favourably with the conventional processes.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalBiotechnology Letters
Issue number2
Publication statusPublished - 1 Feb 2009


  • Antimicrobial activity
  • Biosurfactant
  • Fermentation
  • High-throughput
  • Microbioreactor
  • Pyocyanin


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