Dialysis and gel filtration of isolated low density lipoproteins do not cause a significant loss of low density lipoprotein tocopherol and carotenoid concentration

Mridula Chopra, P. Fitzsimons, M. Hopkins, D. Thurnham

Research output: Contribution to journalArticlepeer-review

Abstract

The resistance of isolated low density lipoprotein (LDL) to copper-initiated oxidation is often used as a measure of effectiveness of an antioxidant intervention. Prior to oxidation excess salt and EDTA are removed via dialysis or gel filtration of the LDL sample. However, there is concern over whether the antioxidant content of dialyzed or gel-filtered LDL is truly representative of native LDL extracted from a blood sample. Previously, the experiments done after the storage of native and dialyzed LDL at −80°C showed that the dialysis step can cause a loss of up to 60% in the tocopherol and carotenoid content of LDL. In the present study, a comparison of the micronutrient concentration in freshly prepared dialyzed and native LDL from 35 subjects showed that after the correction for cholesterol, only lycopene (13%, P
Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalLipids
Volume36
Issue number2
DOIs
Publication statusPublished - Feb 2001

Fingerprint

Dive into the research topics of 'Dialysis and gel filtration of isolated low density lipoproteins do not cause a significant loss of low density lipoprotein tocopherol and carotenoid concentration'. Together they form a unique fingerprint.

Cite this