Differential distribution of nitric oxide synthase between cell fractions isolated from the rat gastric mucosa

J. F. Brown; B. L. Tepperman; P. J. Hanson; B. J. R. Whittle; S. Moncada

doi:10.1016/0006-291X(92)90643-Y

Differential distribution of nitric oxide synthase between cell fractions isolated from the rat gastric mucosa

J. F. Brown, B. L. Tepperman, P. J. Hanson, B. J. R. Whittle^*, S. Moncada

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Cells isolated from the rat gastric mucosa were resolved into two fractions on a percoll density gradient, and into five fractions using counterflow centrifugation (elutriation). Ca²⁺-dependent nitric oxide synthase (NOS) activity was found in the high density percoll fraction but not in the parietal cell enriched low density fraction. This activity was inhibited by N^G-monomethyl-L-arginine with an IC₅₀ of 3.7μM. Cells in the elutriator fraction rich in mucous-epithelial cells exhibited the highest NOS activity, while the smaller cell fractions had no detectable NOS yet had the highest basal release of prostaglandin E₂. The parietal cell enriched elutriator fraction again had low NOS activity. The activity of a constitutive NOS in the mucous-cell fraction may indicate a role of NO in the regulation of epithelial cell integrity or secretion.

Original language	English
Pages (from-to)	680-685
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	184
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)90643-Y
Publication status	Published - 30 Apr 1992

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title = "Differential distribution of nitric oxide synthase between cell fractions isolated from the rat gastric mucosa",

abstract = "Cells isolated from the rat gastric mucosa were resolved into two fractions on a percoll density gradient, and into five fractions using counterflow centrifugation (elutriation). Ca2+-dependent nitric oxide synthase (NOS) activity was found in the high density percoll fraction but not in the parietal cell enriched low density fraction. This activity was inhibited by NG-monomethyl-L-arginine with an IC50 of 3.7μM. Cells in the elutriator fraction rich in mucous-epithelial cells exhibited the highest NOS activity, while the smaller cell fractions had no detectable NOS yet had the highest basal release of prostaglandin E2. The parietal cell enriched elutriator fraction again had low NOS activity. The activity of a constitutive NOS in the mucous-cell fraction may indicate a role of NO in the regulation of epithelial cell integrity or secretion.",

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AU - Brown, J. F.

AU - Tepperman, B. L.

AU - Hanson, P. J.

AU - Whittle, B. J. R.

AU - Moncada, S.

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N2 - Cells isolated from the rat gastric mucosa were resolved into two fractions on a percoll density gradient, and into five fractions using counterflow centrifugation (elutriation). Ca2+-dependent nitric oxide synthase (NOS) activity was found in the high density percoll fraction but not in the parietal cell enriched low density fraction. This activity was inhibited by NG-monomethyl-L-arginine with an IC50 of 3.7μM. Cells in the elutriator fraction rich in mucous-epithelial cells exhibited the highest NOS activity, while the smaller cell fractions had no detectable NOS yet had the highest basal release of prostaglandin E2. The parietal cell enriched elutriator fraction again had low NOS activity. The activity of a constitutive NOS in the mucous-cell fraction may indicate a role of NO in the regulation of epithelial cell integrity or secretion.

AB - Cells isolated from the rat gastric mucosa were resolved into two fractions on a percoll density gradient, and into five fractions using counterflow centrifugation (elutriation). Ca2+-dependent nitric oxide synthase (NOS) activity was found in the high density percoll fraction but not in the parietal cell enriched low density fraction. This activity was inhibited by NG-monomethyl-L-arginine with an IC50 of 3.7μM. Cells in the elutriator fraction rich in mucous-epithelial cells exhibited the highest NOS activity, while the smaller cell fractions had no detectable NOS yet had the highest basal release of prostaglandin E2. The parietal cell enriched elutriator fraction again had low NOS activity. The activity of a constitutive NOS in the mucous-cell fraction may indicate a role of NO in the regulation of epithelial cell integrity or secretion.

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Differential distribution of nitric oxide synthase between cell fractions isolated from the rat gastric mucosa

Abstract

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