@book{f63b3c5cccb64ec494496e8024b18e52,
title = "DNA Manipulation and Analysis",
abstract = "This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.",
editor = "Garry Scarlett",
year = "2023",
month = mar,
day = "1",
doi = "10.1007/978-1-0716-3004-4",
language = "English",
isbn = "9781071630037",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
address = "United States",
edition = "1st",
}