DNA triple-helix formation at target sites containing duplex mismatches

David A. Rusling, Tom Brown, Keith R. Fox

Research output: Contribution to journalArticlepeer-review

Abstract

We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices that contain all 64 N.XZ triplet combinations at the centre (where N, X and Z are each of the four natural DNA bases in turn). Similar experiments were also performed with N=bis-amino-U (BAU) (for stable recognition of AT base pairs) and N=S (for recognition of TA inversions). We find that the introduction of a duplex mismatch destabilises the C+.GZ, T.AZ and G.TZ triplets. A similar effect is seen with BAU.AZ triplets. In contrast, other base combinations, based on non-standard triplets such as C.AZ, T.TZ, G.CZ and A.CZ are stabilised by the presence of a duplex mismatch. In each case S binds to sites containing duplex mismatches better than the corresponding Watson-Crick base pairs.

Original languageEnglish
Pages (from-to)134-140
Number of pages7
JournalBiophysical Chemistry
Volume123
Issue number2-3
Early online date2 Jun 2006
DOIs
Publication statusPublished - 20 Sep 2006

Keywords

  • Base Pair Mismatch
  • Binding Sites
  • DNA/chemistry
  • Hydrogen Bonding
  • Molecular Conformation
  • Nucleic Acid Conformation
  • Oligonucleotides/chemistry
  • Temperature

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