Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids

J. Shiea, H. Min-Zong, H. Hsiu-Jung, L. Chi-Yang, Y. Cheng-Hui, I. Beech, J. Sunner

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds.
    Original languageEnglish
    Pages (from-to)3701-3704
    Number of pages4
    JournalRapid Communications in Mass Spectrometry
    Volume19
    Issue number24
    DOIs
    Publication statusPublished - 2005

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