Electrospray mass spectrometric characterization of the components of protein mixtures and its application to members of the chemokine family of interleukins

I. Kay; S. Hafizi; R. M. Barr; A. I. Mallet; M. W. Greaves

doi:10.1002/bms.1200230505

Electrospray mass spectrometric characterization of the components of protein mixtures and its application to members of the chemokine family of interleukins

I. Kay^*, S. Hafizi, R. M. Barr, A. I. Mallet, M. W. Greaves

^*Corresponding author for this work

School of Pharmacy & Biomedical Sciences

King's College London

Research output: Contribution to journal › Article › peer-review

Abstract

Electrospray mass spectrometry has been employed to detect and characterize the members of a closely related family of immunologically active proteins (chemokines) produced in cell culture by stimulated fibroblasts. The reliability of the method to produce precise data concerning the relative proportions of proteins present in mixtures was investigated in a model system and found to be satisfactory in a range of 10:1 in concentration.

Original language	English
Pages (from-to)	267-271
Number of pages	5
Journal	Biological Mass Spectrometry
Volume	23
Issue number	5
DOIs	https://doi.org/10.1002/bms.1200230505
Publication status	Published - May 1994

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title = "Electrospray mass spectrometric characterization of the components of protein mixtures and its application to members of the chemokine family of interleukins",

abstract = "Electrospray mass spectrometry has been employed to detect and characterize the members of a closely related family of immunologically active proteins (chemokines) produced in cell culture by stimulated fibroblasts. The reliability of the method to produce precise data concerning the relative proportions of proteins present in mixtures was investigated in a model system and found to be satisfactory in a range of 10:1 in concentration.",

author = "I. Kay and S. Hafizi and Barr, {R. M.} and Mallet, {A. I.} and Greaves, {M. W.}",

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AU - Hafizi, S.

AU - Barr, R. M.

AU - Mallet, A. I.

AU - Greaves, M. W.

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AB - Electrospray mass spectrometry has been employed to detect and characterize the members of a closely related family of immunologically active proteins (chemokines) produced in cell culture by stimulated fibroblasts. The reliability of the method to produce precise data concerning the relative proportions of proteins present in mixtures was investigated in a model system and found to be satisfactory in a range of 10:1 in concentration.

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JO - Biological Mass Spectrometry

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Electrospray mass spectrometric characterization of the components of protein mixtures and its application to members of the chemokine family of interleukins

Abstract

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