Manufacturing natural based high-performance composites is becoming a greater interest to the composite manufacturers and to their end users due to their bio-degredability, low cost and availability. Yarn based textile architecture is commonly used in manufacturing these composites due to their excellent formability. However, for using natural based yarn as a reinforcing architectures in high load bearing structural composite applications, a significant improvement in mechanical performance is required. Particularly, jute fibre yarn suffers with poor mechanical properties due to the presence of fibrillar network, polysacharides and other impurities in the fibre. For achieving this, we use aqueous glycine treatment (10%, W/V) on alkali (0.5 %, W/V) and untreated jute yarns for the first time. The glycine treatment on alkali treated jute yarns (ATG) shows a huge improvement in tensile strength and strain values by almost ⁓105% and ⁓50 % respectively compared to untreated jute yarns (UT) because of the strong interactions and bonds developed between glycine, alkali and jute yarns. It is believed that the newly developed glycine treated jute yarns will be helpful to promote jute yarns in composite industries where load-bearing is primary requirement and replace their synthetic counterparts.