Flower-specific KNOX phenotype in the orchid Dactylorhiza fuchsii

Mathew S. Box, Steven Dodsworth, Paula J. Rudall, Richard M. Bateman, Beverley J. Glover*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Downloads (Pure)

Abstract

The KNOTTED1-like homeobox (KNOX) genes are best known for maintaining a pluripotent stem-cell population in the shoot apical meristem that underlies indeterminate vegetative growth, allowing plants to adapt their development to suit the prevailing environmental conditions. More recently, the function of the KNOX gene family has been expanded to include additional roles in lateral organ development such as complex leaf morphogenesis, which has come to dominate the KNOX literature. Despite several reports implicating KNOX genes in the development of carpels and floral elaborations such as petal spurs, few authors have investigated the role of KNOX genes in flower development. Evidence is presented here of a flower-specific KNOX function in the development of the elaborate flowers of the orchid Dactylorhiza fuchsii, which have a three-lobed labellum petal with a prominent spur. Using degenerate PCR, four Class I KNOX genes (DfKN14) have been isolated, one from each of the four major Class I KNOX subclades and by reverse transcription PCR (RT-PCR), it is demonstrated that DfKNOX transcripts are detectable in developing floral organs such as the spur-bearing labellum and inferior ovary. Although constitutive expression of the DfKN2 transcript in tobacco produces a wide range of floral abnormalities, including serrated petal margins, extra petal tissue, and fused organs, none of the vegetative phenotypes typical of constitutive KNOX expression were produced. These data are highly suggestive of a role for KNOX expression in floral development that may be especially important in taxa with elaborate flowers.

Original languageEnglish
Pages (from-to)4811-4819
Number of pages9
JournalJournal of Experimental Botany
Volume63
Issue number13
Early online date5 Jul 2012
DOIs
Publication statusPublished - 1 Aug 2012

Keywords

  • Dactylorhiza fuchsii
  • evolution
  • flower development
  • KNOX genes
  • labellum
  • orchids
  • petal shape
  • petal spur

Fingerprint

Dive into the research topics of 'Flower-specific KNOX phenotype in the orchid Dactylorhiza fuchsii'. Together they form a unique fingerprint.

Cite this