Fluorous-directed assembly of DNA origami nanostructures

Jiajia Zou; Ashley C. Stammers; Andrea Taladriz-Sender; Jamie M. Withers; Iain Christie; Marina Santana Vega; Badri L. Aekbote; William J. Peveler; David A. Rusling; Glenn A. Burley; Alasdair W. Clark

doi:10.1021/acsnano.2c10727

Fluorous-directed assembly of DNA origami nanostructures

Jiajia Zou, Ashley C. Stammers, Andrea Taladriz-Sender, Jamie M. Withers, Iain Christie, Marina Santana Vega, Badri L. Aekbote, William J. Peveler, David A. Rusling, Glenn A. Burley, Alasdair W. Clark

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Abstract

An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated "catch-and-latch" approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.

Original language	English
Pages (from-to)	752-759
Number of pages	8
Journal	ACS Nano
Volume	17
Issue number	1
Early online date	20 Dec 2022
DOIs	https://doi.org/10.1021/acsnano.2c10727
Publication status	Published - 10 Jan 2023

Keywords

DNA nanotechnology
DNA origami
DNA origami dimerization
fluorous
fluorous DNA
molecular recognition
self-assembly
UKRI
BBSRC
BB/T000627/1
BB/N016734/1
EPSRC
EP/ V030515/1

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10.1021/acsnano.2c10727

Fluorous-directed-assembly-of-dna-origami-nanostructures FINALFinal published version, 6.68 MBLicence: CC BY

Cite this

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title = "Fluorous-directed assembly of DNA origami nanostructures",

abstract = "An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated {"}catch-and-latch{"} approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.",

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author = "Jiajia Zou and Stammers, {Ashley C.} and Andrea Taladriz-Sender and Withers, {Jamie M.} and Iain Christie and {Santana Vega}, Marina and Aekbote, {Badri L.} and Peveler, {William J.} and Rusling, {David A.} and Burley, {Glenn A.} and Clark, {Alasdair W.}",

note = "Funding Information: This work was supported by The Leverhulme Trust (Grant No. RPG-2018-149), the BBSRC (Grant Nos. BB/T000627/1 & BB/N016734/1), and the EPSRC (Grant No. EP/V030515/1). The authors also wish to thank all the staff working in the James Watt Nanofabrication Centre for their support. Publisher Copyright: {\textcopyright} 2022 The Authors. Published by American Chemical Society.",

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doi = "10.1021/acsnano.2c10727",

language = "English",

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AU - Zou, Jiajia

AU - Stammers, Ashley C.

AU - Taladriz-Sender, Andrea

AU - Withers, Jamie M.

AU - Christie, Iain

AU - Santana Vega, Marina

AU - Aekbote, Badri L.

AU - Peveler, William J.

AU - Rusling, David A.

AU - Burley, Glenn A.

AU - Clark, Alasdair W.

N1 - Funding Information: This work was supported by The Leverhulme Trust (Grant No. RPG-2018-149), the BBSRC (Grant Nos. BB/T000627/1 & BB/N016734/1), and the EPSRC (Grant No. EP/V030515/1). The authors also wish to thank all the staff working in the James Watt Nanofabrication Centre for their support. Publisher Copyright: © 2022 The Authors. Published by American Chemical Society.

PY - 2023/1/10

Y1 - 2023/1/10

N2 - An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated "catch-and-latch" approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.

AB - An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated "catch-and-latch" approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.

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Fluorous-directed assembly of DNA origami nanostructures

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Keywords

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