Fluorous-Directed Clamping Stabilizes Triple-Helical DNA

Andrea Taladriz-Sender; Michael Brazzill; Jamie M. Withers; Alasdair W. Clark; Glenn A. Burley; David A. Rusling

doi:10.1021/acsomega.6c00803

Fluorous-Directed Clamping Stabilizes Triple-Helical DNA

Andrea Taladriz-Sender
, Michael Brazzill
, Jamie M. Withers
, Alasdair W. Clark
, Glenn A. Burley
, David A. Rusling

Research output: Contribution to journal › Article › peer-review

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Abstract

Here we present a strategy to stabilize DNA triplexes by the attachment of perfluorinated tails to the 3' and/or 5' end of oligodeoxyribonucleotides. The tails preferentially associate with one another via the "fluorous effect" without compromising sequence selectivity. The most stable complex resulted in a dramatic 14 °C increase in melting temperature at neutral pH. This orthogonal recognition approach broadens the applications of triplex DNA in diagnostics, therapy, and DNA nanotechnology.

Original language	English
Pages (from-to)	33983-33990
Number of pages	8
Journal	ACS Omega
Volume	11
Issue number	23
DOIs	https://doi.org/10.1021/acsomega.6c00803
Publication status	Published - 1 Jun 2026

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Fluorous-Directed Clamping Stabilizes Triple-Helical DNAFinal published version, 3.16 MBLicence: CC BY

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abstract = "Here we present a strategy to stabilize DNA triplexes by the attachment of perfluorinated tails to the 3' and/or 5' end of oligodeoxyribonucleotides. The tails preferentially associate with one another via the {"}fluorous effect{"} without compromising sequence selectivity. The most stable complex resulted in a dramatic 14 °C increase in melting temperature at neutral pH. This orthogonal recognition approach broadens the applications of triplex DNA in diagnostics, therapy, and DNA nanotechnology.",

author = "Andrea Taladriz-Sender and Michael Brazzill and Withers, \{Jamie M.\} and Clark, \{Alasdair W.\} and Burley, \{Glenn A.\} and Rusling, \{David A.\}",

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AU - Taladriz-Sender, Andrea

AU - Brazzill, Michael

AU - Withers, Jamie M.

AU - Clark, Alasdair W.

AU - Burley, Glenn A.

AU - Rusling, David A.

PY - 2026/6/1

Y1 - 2026/6/1

N2 - Here we present a strategy to stabilize DNA triplexes by the attachment of perfluorinated tails to the 3' and/or 5' end of oligodeoxyribonucleotides. The tails preferentially associate with one another via the "fluorous effect" without compromising sequence selectivity. The most stable complex resulted in a dramatic 14 °C increase in melting temperature at neutral pH. This orthogonal recognition approach broadens the applications of triplex DNA in diagnostics, therapy, and DNA nanotechnology.

AB - Here we present a strategy to stabilize DNA triplexes by the attachment of perfluorinated tails to the 3' and/or 5' end of oligodeoxyribonucleotides. The tails preferentially associate with one another via the "fluorous effect" without compromising sequence selectivity. The most stable complex resulted in a dramatic 14 °C increase in melting temperature at neutral pH. This orthogonal recognition approach broadens the applications of triplex DNA in diagnostics, therapy, and DNA nanotechnology.

U2 - 10.1021/acsomega.6c00803

DO - 10.1021/acsomega.6c00803

M3 - Article

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VL - 11

SP - 33983

EP - 33990

JO - ACS Omega

JF - ACS Omega

IS - 23

ER -

Fluorous-Directed Clamping Stabilizes Triple-Helical DNA

Abstract

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