Generation of functional-RNA arrays by in vitro transcription and in situ RNA capture for the detection of RNA-RNA interactions

Helen Vincent, Charlotte Henderson, Daniela Lopes Cardoso, Anastasia Callaghan

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review


RNA performs a wide variety of vital cellular functions. These functions typically require interactions with other biological macromolecules, often as part of an intricate communication network. High-throughput techniques capable of analyzing RNA-based interactions are therefore essential. Functional-RNA arrays address this need, providing the capability of performing hundreds of miniature assays in parallel. Here we describe a method to generate functional-RNA arrays using in vitro transcription of a DNA template array and in situ RNA capture. We also suggest how functional-RNA arrays could be applied to investigating RNA-RNA interactions.
Original languageEnglish
Title of host publicationDNA Manipulation and Analysis
EditorsGarry Scarlett
PublisherHumana Press
Number of pages22
ISBN (Electronic)9781071630044
ISBN (Print)9781071630037
Publication statusPublished - 1 Mar 2023

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • RNA array
  • RNA binding protein
  • RNA−RNA interactions
  • Surface Immobilization
  • In vitro
  • Transcription
  • In situ capture
  • Microarrays

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