Green biogenic fabrication of AgNPs derived from Pulicaria jaubertii leaf extract: Investigation of antimicrobial and cytotoxic properties

"Plant-mediated synthesis of nanomaterials offers an eco-friendly and cost-effective alternative for biomedical applications. In this study, silver nanoparticles (AgNPs) were green-synthesized using a leaf extract of Pulicaria jaubertii, which served as both a reducing and stabilizing agent. The biophysical characterization of synthesized AgNPs confirmed their successful fabrication through Ultraviolet-Visible spectroscopy, Dynamic Light Scattering, Fourier Transform Infrared spectroscopy, Transmission Electron Microscopy, and Scanning Electron Microscope- Energy Dispersive X-ray Analysis. The synthesized AgNPs demonstrated potent antibacterial activity against gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) pathogens, as evidenced by agar well diffusion, time-kill, and antibiofilm assays. Notably, the nanoparticles exhibited minimal cytotoxicity toward Peripheral Blood Mononuclear Cells and Human Red Blood Cells, underscoring their biocompatibility. These findings highlight the potential of Pulicaria jaubertii-derived AgNPs as promising candidates for antimicrobial therapies and biomedical applications."