TY - JOUR
T1 - Gut commensal E. coli proteins activate host satiety pathways following nutrient-induced bacterial growth
AU - Brenton, Jonathan
AU - Tennoune, Naouel
AU - Lucas, Nicolas
AU - Francois, Marie
AU - Legrand, Romain
AU - Jacquemot, Justine
AU - Goichon, Alexis
AU - Guerin, Charlene
AU - Peltier, Johann
AU - Pestel-Caron, Martine
AU - Chan, Philippe
AU - Vaudry, David
AU - do Rego, Jean-Claude
AU - Lienard, Fabienne
AU - Penicaud, Luc
AU - Fioramonti, Xavier
AU - Ebenezer, Ivor
AU - Hokfelt, Tomas
AU - Dechelotte, Pierre
AU - Fetissov, Serguei
PY - 2016/2/9
Y1 - 2016/2/9
N2 - The composition of gut microbiota has been associated with host metabolic phenotypes, but it is not known if gut bacteria may influence host appetite. Here we show that regular nutrient provision stabilizes exponential growth of E. coli, with the stationary phase occurring 20 min after nutrient supply accompanied by bacterial proteome changes, suggesting involvement of bacterial proteins in host satiety. Indeed, intestinal infusions of E. coli stationary phase proteins increased plasma PYY and their intraperitoneal injections suppressed acutely food intake and activated c-Fos in hypothalamic POMC neurons, while their repeated administrations reduced meal size. ClpB, a bacterial protein mimetic of α-MSH, was upregulated in the E. coli stationary phase, was detected in plasma proportional to ClpB DNA in feces, and stimulated firing rate of hypothalamic POMC neurons. Thus, these data show that bacterial proteins produced after nutrient-induced E. coli growth may signal meal termination. Furthermore, continuous exposure to E. coli proteins may influence long-term meal pattern.
AB - The composition of gut microbiota has been associated with host metabolic phenotypes, but it is not known if gut bacteria may influence host appetite. Here we show that regular nutrient provision stabilizes exponential growth of E. coli, with the stationary phase occurring 20 min after nutrient supply accompanied by bacterial proteome changes, suggesting involvement of bacterial proteins in host satiety. Indeed, intestinal infusions of E. coli stationary phase proteins increased plasma PYY and their intraperitoneal injections suppressed acutely food intake and activated c-Fos in hypothalamic POMC neurons, while their repeated administrations reduced meal size. ClpB, a bacterial protein mimetic of α-MSH, was upregulated in the E. coli stationary phase, was detected in plasma proportional to ClpB DNA in feces, and stimulated firing rate of hypothalamic POMC neurons. Thus, these data show that bacterial proteins produced after nutrient-induced E. coli growth may signal meal termination. Furthermore, continuous exposure to E. coli proteins may influence long-term meal pattern.
U2 - 10.1016/j.cmet.2015.10.017
DO - 10.1016/j.cmet.2015.10.017
M3 - Article
SN - 1932-7420
VL - 23
SP - 324
EP - 334
JO - Cell Metabolism
JF - Cell Metabolism
ER -