High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro

Chris Read; Anne Marie Larose; Benoît Leblanc; Andrew J. Bannister; Simon Firek; Duncan R. Smith; Tom Moss

High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro

Chris Read, Anne Marie Larose, Benoît Leblanc, Andrew J. Bannister, Simon Firek, Duncan R. Smith, Tom Moss^*

^*Corresponding author for this work

Institute of Life Sciences and Healthcare

Research output: Contribution to journal › Article › peer-review

Abstract

The first high resolution maps of the Xenopus laevis ribosomal promoter and its flanking regions (-179 to +14) have been created by assaying point mutants both in oocyte and in vitro. Within the promoter boundaries (-141(-145) to +3(+4)), domains analogous to the Core Promoter and "Upstream Control Element" (UCE) were clearly detected. The base pairs at -133, within the UCE, and -20, -10, -7, and +3, within the Core, were all shown to be especially important for promotion. Between the Core and UCE, two central promoter elements (CPEs) were also resolved. Surprisingly, these CPEs did not correspond to the highly conserved enhancer homology (∼-70 to -110), but to CCCGGCC motifs immediately flanking it. Although xUBF was shown to be a limiting component for in vitro transcription, none of the point mutations was found to affect the interaction of this factor with the promoter.

Original language	English
Pages (from-to)	10961-10967
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	267
Issue number	16
Publication status	Published - 5 Jun 1992

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title = "High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro",

abstract = "The first high resolution maps of the Xenopus laevis ribosomal promoter and its flanking regions (-179 to +14) have been created by assaying point mutants both in oocyte and in vitro. Within the promoter boundaries (-141(-145) to +3(+4)), domains analogous to the Core Promoter and {"}Upstream Control Element{"} (UCE) were clearly detected. The base pairs at -133, within the UCE, and -20, -10, -7, and +3, within the Core, were all shown to be especially important for promotion. Between the Core and UCE, two central promoter elements (CPEs) were also resolved. Surprisingly, these CPEs did not correspond to the highly conserved enhancer homology (∼-70 to -110), but to CCCGGCC motifs immediately flanking it. Although xUBF was shown to be a limiting component for in vitro transcription, none of the point mutations was found to affect the interaction of this factor with the promoter.",

author = "Chris Read and Larose, {Anne Marie} and Beno{\^i}t Leblanc and Bannister, {Andrew J.} and Simon Firek and Smith, {Duncan R.} and Tom Moss",

year = "1992",

month = jun,

day = "5",

language = "English",

volume = "267",

pages = "10961--10967",

journal = "Journal of Biological Chemistry",

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T1 - High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro

AU - Read, Chris

AU - Larose, Anne Marie

AU - Leblanc, Benoît

AU - Bannister, Andrew J.

AU - Firek, Simon

AU - Smith, Duncan R.

AU - Moss, Tom

PY - 1992/6/5

Y1 - 1992/6/5

N2 - The first high resolution maps of the Xenopus laevis ribosomal promoter and its flanking regions (-179 to +14) have been created by assaying point mutants both in oocyte and in vitro. Within the promoter boundaries (-141(-145) to +3(+4)), domains analogous to the Core Promoter and "Upstream Control Element" (UCE) were clearly detected. The base pairs at -133, within the UCE, and -20, -10, -7, and +3, within the Core, were all shown to be especially important for promotion. Between the Core and UCE, two central promoter elements (CPEs) were also resolved. Surprisingly, these CPEs did not correspond to the highly conserved enhancer homology (∼-70 to -110), but to CCCGGCC motifs immediately flanking it. Although xUBF was shown to be a limiting component for in vitro transcription, none of the point mutations was found to affect the interaction of this factor with the promoter.

AB - The first high resolution maps of the Xenopus laevis ribosomal promoter and its flanking regions (-179 to +14) have been created by assaying point mutants both in oocyte and in vitro. Within the promoter boundaries (-141(-145) to +3(+4)), domains analogous to the Core Promoter and "Upstream Control Element" (UCE) were clearly detected. The base pairs at -133, within the UCE, and -20, -10, -7, and +3, within the Core, were all shown to be especially important for promotion. Between the Core and UCE, two central promoter elements (CPEs) were also resolved. Surprisingly, these CPEs did not correspond to the highly conserved enhancer homology (∼-70 to -110), but to CCCGGCC motifs immediately flanking it. Although xUBF was shown to be a limiting component for in vitro transcription, none of the point mutations was found to affect the interaction of this factor with the promoter.

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 16

ER -

High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro

Abstract

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