Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells

H. Ross; Helen Fillmore

doi:10.1016/j.febslet.2007.11.074

Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells

Research output: Contribution to journal › Article › peer-review

Abstract

Membrane-type 5 matrix metalloproteinase (MT5-MMP) expression is ubiquitous in brain development while restricted to regions of neuroplasticity in adult. In the multipotent NT2 model of neurogenesis and differentiation, MT5-MMP is differentially expressed with significantly higher mRNA levels in the differentiated neuronal hNT cells. MT5-MMP cDNA cloned from NT2 cells unexpectedly revealed a novel sequence (MT5-MMPvar) characterized by a 162 bp deletion. Both transcripts were identified in NT2, hNT and adult human hippocampus. In vitro, MT5-MMPvar translated into a ∼55 kDa protein. It was also detected in NT2, hNT and adult human hippocampus. These results suggest more than one human MT5-MMP transcript may exist in the central nervous system.

Original language	English
Pages (from-to)	5923-5928
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	30
DOIs	https://doi.org/10.1016/j.febslet.2007.11.074
Publication status	Published - 22 Dec 2007

Access to Document

10.1016/j.febslet.2007.11.074

Cite this

@article{c452d3820bc948ef86d6954f16897eaf,

title = "Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells",

abstract = "Membrane-type 5 matrix metalloproteinase (MT5-MMP) expression is ubiquitous in brain development while restricted to regions of neuroplasticity in adult. In the multipotent NT2 model of neurogenesis and differentiation, MT5-MMP is differentially expressed with significantly higher mRNA levels in the differentiated neuronal hNT cells. MT5-MMP cDNA cloned from NT2 cells unexpectedly revealed a novel sequence (MT5-MMPvar) characterized by a 162 bp deletion. Both transcripts were identified in NT2, hNT and adult human hippocampus. In vitro, MT5-MMPvar translated into a ∼55 kDa protein. It was also detected in NT2, hNT and adult human hippocampus. These results suggest more than one human MT5-MMP transcript may exist in the central nervous system.",

author = "H. Ross and Helen Fillmore",

year = "2007",

month = dec,

day = "22",

doi = "10.1016/j.febslet.2007.11.074",

language = "English",

volume = "581",

pages = "5923--5928",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "30",

}

TY - JOUR

T1 - Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells

AU - Ross, H.

AU - Fillmore, Helen

PY - 2007/12/22

Y1 - 2007/12/22

N2 - Membrane-type 5 matrix metalloproteinase (MT5-MMP) expression is ubiquitous in brain development while restricted to regions of neuroplasticity in adult. In the multipotent NT2 model of neurogenesis and differentiation, MT5-MMP is differentially expressed with significantly higher mRNA levels in the differentiated neuronal hNT cells. MT5-MMP cDNA cloned from NT2 cells unexpectedly revealed a novel sequence (MT5-MMPvar) characterized by a 162 bp deletion. Both transcripts were identified in NT2, hNT and adult human hippocampus. In vitro, MT5-MMPvar translated into a ∼55 kDa protein. It was also detected in NT2, hNT and adult human hippocampus. These results suggest more than one human MT5-MMP transcript may exist in the central nervous system.

AB - Membrane-type 5 matrix metalloproteinase (MT5-MMP) expression is ubiquitous in brain development while restricted to regions of neuroplasticity in adult. In the multipotent NT2 model of neurogenesis and differentiation, MT5-MMP is differentially expressed with significantly higher mRNA levels in the differentiated neuronal hNT cells. MT5-MMP cDNA cloned from NT2 cells unexpectedly revealed a novel sequence (MT5-MMPvar) characterized by a 162 bp deletion. Both transcripts were identified in NT2, hNT and adult human hippocampus. In vitro, MT5-MMPvar translated into a ∼55 kDa protein. It was also detected in NT2, hNT and adult human hippocampus. These results suggest more than one human MT5-MMP transcript may exist in the central nervous system.

U2 - 10.1016/j.febslet.2007.11.074

DO - 10.1016/j.febslet.2007.11.074

M3 - Article

SN - 0014-5793

VL - 581

SP - 5923

EP - 5928

JO - FEBS Letters

JF - FEBS Letters

IS - 30

ER -

Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells

Abstract

Access to Document

Fingerprint

Cite this