Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells

H. Ross; Helen Fillmore

doi:10.1016/j.febslet.2007.11.074

Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells

H. Ross, Helen Fillmore

Research output: Contribution to journal › Article › peer-review

Abstract

Membrane-type 5 matrix metalloproteinase (MT5-MMP) expression is ubiquitous in brain development while restricted to regions of neuroplasticity in adult. In the multipotent NT2 model of neurogenesis and differentiation, MT5-MMP is differentially expressed with significantly higher mRNA levels in the differentiated neuronal hNT cells. MT5-MMP cDNA cloned from NT2 cells unexpectedly revealed a novel sequence (MT5-MMPvar) characterized by a 162 bp deletion. Both transcripts were identified in NT2, hNT and adult human hippocampus. In vitro, MT5-MMPvar translated into a ∼55 kDa protein. It was also detected in NT2, hNT and adult human hippocampus. These results suggest more than one human MT5-MMP transcript may exist in the central nervous system.

Original language	English
Pages (from-to)	5923-5928
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	30
DOIs	https://doi.org/10.1016/j.febslet.2007.11.074
Publication status	Published - 22 Dec 2007

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10.1016/j.febslet.2007.11.074

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title = "Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells",

abstract = "Membrane-type 5 matrix metalloproteinase (MT5-MMP) expression is ubiquitous in brain development while restricted to regions of neuroplasticity in adult. In the multipotent NT2 model of neurogenesis and differentiation, MT5-MMP is differentially expressed with significantly higher mRNA levels in the differentiated neuronal hNT cells. MT5-MMP cDNA cloned from NT2 cells unexpectedly revealed a novel sequence (MT5-MMPvar) characterized by a 162 bp deletion. Both transcripts were identified in NT2, hNT and adult human hippocampus. In vitro, MT5-MMPvar translated into a ∼55 kDa protein. It was also detected in NT2, hNT and adult human hippocampus. These results suggest more than one human MT5-MMP transcript may exist in the central nervous system.",

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doi = "10.1016/j.febslet.2007.11.074",

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AU - Ross, H.

AU - Fillmore, Helen

PY - 2007/12/22

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AB - Membrane-type 5 matrix metalloproteinase (MT5-MMP) expression is ubiquitous in brain development while restricted to regions of neuroplasticity in adult. In the multipotent NT2 model of neurogenesis and differentiation, MT5-MMP is differentially expressed with significantly higher mRNA levels in the differentiated neuronal hNT cells. MT5-MMP cDNA cloned from NT2 cells unexpectedly revealed a novel sequence (MT5-MMPvar) characterized by a 162 bp deletion. Both transcripts were identified in NT2, hNT and adult human hippocampus. In vitro, MT5-MMPvar translated into a ∼55 kDa protein. It was also detected in NT2, hNT and adult human hippocampus. These results suggest more than one human MT5-MMP transcript may exist in the central nervous system.

U2 - 10.1016/j.febslet.2007.11.074

DO - 10.1016/j.febslet.2007.11.074

M3 - Article

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SP - 5923

EP - 5928

JO - FEBS Letters

JF - FEBS Letters

IS - 30

ER -

Identification of a novel human MT5-MMP transcript variant in multipotent NT2 cells

Abstract

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