Third Stream Activity (TSA) is increasingly important to UK Universities and the wider economy, through innovation and entrepreneurship. Using data from the 2009/10 UK Higher Education Business and Community Interaction Survey (HE-BCIS) this study investigates UK universities’ TSA. Through considering the data in original and logged forms, two interpretations of TSA are investigated, in relation to entrepreneurial and enterprising university concepts. Using Principle Component Analysis (PCA) on both data forms, four factors relating to universities’ TSA are identified. A nascent indexing approach is employed to create sub-indexes using the identified factors, weight aggregated to produce final TSA indexes (one for each form of the data). Comparisons are then made between rankings of universities using the two versions of TSA index, and sub-indexes, illustrating differences utilising the entrepreneurial and enterprising university concepts. Important questions are raised for future government policy in terms of promoting interventions that drive towards different TSA types.