Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response

E. Thian; J. Huang; Zeeshan Ahmad; M. Edirisinghe; S. Jayasinghe; D. Ireland; R. Brooks; N. Rushton; S. Best; W. Bonfield

doi:10.1002/jbm.a.31564

Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response

E. Thian, J. Huang, Zeeshan Ahmad, M. Edirisinghe, S. Jayasinghe, D. Ireland, R. Brooks, N. Rushton, S. Best, W. Bonfield

Research output: Contribution to journal › Article › peer-review

Abstract

Electrohydrodynamic spraying has been used to produce patterns of line width up to 100 lm in size on glass discs, using nanohydroxyapatite (nHA). A human osteoblast (HOB)-like cell model was then used to study the interaction between the HOB cells and nHA patterns in vitro. Growth of the cells was significantly increased (p < 0.05) on the nHA surfaces. In addition, HOBs attached and spread well, secreting extracellular matrix. It was found that a confluent, aligned cell layer was achieved on nHA patterns by day 9. Immunofluorescent staining indicated that these cells showed elongated nuclei, enhanced adhesion (vinculin adhesion plaques) and a well-aligned cytoskeleton (actin stress fibres). This work suggests that this type of spraying may provide a route for the production of nanoscale features on implants for biomedical applications.

Original language	English
Pages (from-to)	188-194
Number of pages	7
Journal	Journal of Biomedical Materials Research Part A
Volume	85A
Issue number	1
DOIs	https://doi.org/10.1002/jbm.a.31564
Publication status	Published - 2008

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title = "Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response",

abstract = "Electrohydrodynamic spraying has been used to produce patterns of line width up to 100 lm in size on glass discs, using nanohydroxyapatite (nHA). A human osteoblast (HOB)-like cell model was then used to study the interaction between the HOB cells and nHA patterns in vitro. Growth of the cells was significantly increased (p < 0.05) on the nHA surfaces. In addition, HOBs attached and spread well, secreting extracellular matrix. It was found that a confluent, aligned cell layer was achieved on nHA patterns by day 9. Immunofluorescent staining indicated that these cells showed elongated nuclei, enhanced adhesion (vinculin adhesion plaques) and a well-aligned cytoskeleton (actin stress fibres). This work suggests that this type of spraying may provide a route for the production of nanoscale features on implants for biomedical applications.",

author = "E. Thian and J. Huang and Zeeshan Ahmad and M. Edirisinghe and S. Jayasinghe and D. Ireland and R. Brooks and N. Rushton and S. Best and W. Bonfield",

year = "2008",

doi = "10.1002/jbm.a.31564",

language = "English",

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pages = "188--194",

journal = "Journal of Biomedical Materials Research Part A",

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T1 - Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response

AU - Thian, E.

AU - Huang, J.

AU - Ahmad, Zeeshan

AU - Edirisinghe, M.

AU - Jayasinghe, S.

AU - Ireland, D.

AU - Brooks, R.

AU - Rushton, N.

AU - Best, S.

AU - Bonfield, W.

PY - 2008

Y1 - 2008

N2 - Electrohydrodynamic spraying has been used to produce patterns of line width up to 100 lm in size on glass discs, using nanohydroxyapatite (nHA). A human osteoblast (HOB)-like cell model was then used to study the interaction between the HOB cells and nHA patterns in vitro. Growth of the cells was significantly increased (p < 0.05) on the nHA surfaces. In addition, HOBs attached and spread well, secreting extracellular matrix. It was found that a confluent, aligned cell layer was achieved on nHA patterns by day 9. Immunofluorescent staining indicated that these cells showed elongated nuclei, enhanced adhesion (vinculin adhesion plaques) and a well-aligned cytoskeleton (actin stress fibres). This work suggests that this type of spraying may provide a route for the production of nanoscale features on implants for biomedical applications.

AB - Electrohydrodynamic spraying has been used to produce patterns of line width up to 100 lm in size on glass discs, using nanohydroxyapatite (nHA). A human osteoblast (HOB)-like cell model was then used to study the interaction between the HOB cells and nHA patterns in vitro. Growth of the cells was significantly increased (p < 0.05) on the nHA surfaces. In addition, HOBs attached and spread well, secreting extracellular matrix. It was found that a confluent, aligned cell layer was achieved on nHA patterns by day 9. Immunofluorescent staining indicated that these cells showed elongated nuclei, enhanced adhesion (vinculin adhesion plaques) and a well-aligned cytoskeleton (actin stress fibres). This work suggests that this type of spraying may provide a route for the production of nanoscale features on implants for biomedical applications.

U2 - 10.1002/jbm.a.31564

DO - 10.1002/jbm.a.31564

M3 - Article

SN - 1549-3296

VL - 85A

SP - 188

EP - 194

JO - Journal of Biomedical Materials Research Part A

JF - Journal of Biomedical Materials Research Part A

IS - 1

ER -

Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response

Abstract

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