Interaction of serum amyloid P component with hexanoyl bis(D-proline) (CPHPC)

Simon E. Kolstoe, Michelle C. Jenvey, Alan Purvis, Mark E. Light, Darren Thompson, Peter Hughes, Mark B. Pepys, Stephen P. Wood

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Abstract

Under physiological conditions, the pentameric human plasma protein serum amyloid P component (SAP) binds hexanoyl bis(D-proline) (R-1-{6-[R-2-carboxy-pyrrolidin-1-yl]-6-oxo-hexanoyl}pyrrolidine-2-carboxylic acid; CPHPC) through its D-proline head groups in a calcium-dependent interaction. Cooperative effects in binding lead to a substantial enhancement of affinity. Five molecules of the bivalent ligand cross-link and stabilize pairs of SAP molecules, forming a decameric complex that is rapidly cleared from the circulation by the liver. Here, it is reported that X-ray analysis of the SAP complex with CPHPC and cadmium ions provides higher resolution detail of the interaction than is observed with calcium ions. Conformational isomers of CPHPC observed in solution by HPLC and by X-ray analysis are compared with the protein-bound form. These are discussed in relation to the development of CPHPC to provide SAP depletion for the treatment of amyloidosis and other indications.

Original languageEnglish
Pages (from-to)2232-2240
Number of pages9
JournalActa Crystallographica Section D
Volume70
Issue number8
Early online date25 Jul 2014
DOIs
Publication statusPublished - 1 Aug 2014

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