Interferon-γ reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells

Claire Hastie, J. Masters, S. Moss, S. Naaby-Hansen

Research output: Contribution to journalArticlepeer-review


The effect of interferon-γ (IFNγ) treatment on cell surface protein expression was studied in the human prostate cancer cell line 1542CP3TX. IFNγ increased both the number and abundance of proteins in membrane fractions. In contrast, the expression of annexin 2 and its binding partner p11 decreased by 4-fold after 24 h of exposure, with the remaining anx2t complexes localized to lipid rafts. Within the same time scale, IFNγ reduced the abundance of the peripherally attached, anx2t-associated proteases procathepsin B and plasminogen. The invasive capacity of the cancer cells was reduced by treatment with IFNγ or antibody to annexin 2 in 1542CP3TX cells, but not in LNCaP, an annexin 2-negative prostate cancer cell line. Expression of annexin 2 in LNCaP cells increased their invasiveness. IFNγ induced calpain expression and activation and increased the phosphorylation and degradation of the calpain substrate ABCA1 in 1542CP3TX cancer cells. Surface expression of annexin 2 was reduced in cells treated with glyburide, an ABCA1 inhibitor, whereas inhibition of calpain abrogated IFNγ-induced annexin 2 down-regulation and suppression of Matrigel invasion. The findings suggest annexin 2 externalization is coupled to lipid efflux in prostate epithelium and that IFNγ induces down-regulation of the protease-binding anx2t scaffold at the cell surface and consequently acts to suppress invasiveness through calpain-mediated degradation of the lipid transporter ABCA1.
Original languageEnglish
Pages (from-to)12595-12603
Number of pages9
JournalThe Journal of Biological Chemistry
Issue number18
Publication statusPublished - 2012


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