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Isolation and culture of vascular smooth muscle cells from small and large vessels

  • A. C. Montezano
  • , R. A. Lopes
  • , K. B. Neves
  • , F. Rios
  • , R. M. Touyz

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Primary culture of vascular smooth muscle cells is an important in vitro model for the dissection of molecular mechanisms related to a specific physiological or pathological response at the cellular level. Cultured cells also provide an excellent model to study cell biology. This chapter describes a user-friendly and practical protocol for isolation of vascular smooth muscle cells from small and large vessels by enzymatic dissociation, which can be applied to vessels from different species, including rodents and humans.
Original languageEnglish
Title of host publicationHypertension: Methods and Protocols
EditorsRhian M. Touyz, Ernesto L. Schiffrin
PublisherSpringer Nature
Pages349–354
ISBN (Electronic)9781493966257
ISBN (Print)9781493966233, 9781493982530
DOIs
Publication statusPublished - 24 Jan 2017

Publication series

NameMethods in Molecular Biology
Volume1527
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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