Isolation of intact high molecular weight chromosomal DNA from Desulfovibrio spp.

Vitaly Zinkevich, I. Beech

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A simple, reproducible method to extract high quality DNA from sulfate-reducing bacteria (SRB) is described. SRB isolated from patients with ulcerative colitis, and from environmental samples (D. indonensis), were used for purification of chromosomal DNA. DNA yield purified by this method was more than one order of magnitude higher compared with the Marmur method, phenol-chloroform or QIAGEN procedures. A 10 ml culture was enough for isolation of sufficient DNA to perform hundreds of PCR-based reactions, and can be used in other DNA manipulation techniques such as restriction digestion and DNA cloning despite a low yield of cells (1×107-1×108 cells/ml).
    Original languageEnglish
    Pages (from-to)29-33
    Number of pages5
    JournalMolecular Biology Today
    Volume1
    Issue number1
    Publication statusPublished - 2000

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