A simple, reproducible method to extract high quality DNA from sulfate-reducing bacteria (SRB) is described. SRB isolated from patients with ulcerative colitis, and from environmental samples (D. indonensis), were used for purification of chromosomal DNA. DNA yield purified by this method was more than one order of magnitude higher compared with the Marmur method, phenol-chloroform or QIAGEN procedures. A 10 ml culture was enough for isolation of sufficient DNA to perform hundreds of PCR-based reactions, and can be used in other DNA manipulation techniques such as restriction digestion and DNA cloning despite a low yield of cells (1×107-1×108 cells/ml).
|Number of pages||5|
|Journal||Molecular Biology Today|
|Publication status||Published - 2000|