Abstract
A photolyase-like protein gene found in the Trypanosoma cruzi genome database was cloned and expressed in Escherichia coli resulting in the formation of inclusion bodies. Antibodies against this protein were used to determine expression of the protein in the different forms of the parasite. It was visualized in the epimastigote form but not in amastigote or trypomastigote forms obtained from culture in Vero cells. In epimastigotes, this protein is located at the level of the mitochondrion associated to both sides of the kinetoplast. Sequence analyses indicated that this protein, as well as other photolyases from Leishmania spp. and Trypanosoma brucei are related to single-stranded photolyases or cryptochromes DASH.
| Original language | English |
|---|---|
| Pages (from-to) | 350-356 |
| Number of pages | 7 |
| Journal | Experimental Parasitology |
| Volume | 124 |
| Issue number | 3 |
| Early online date | 26 Nov 2009 |
| DOIs | |
| Publication status | Published - Mar 2010 |
Keywords
- Amino Acid Sequence
- Animals
- Antibodies, Protozoan
- Blotting, Western
- Cloning, Molecular
- Cryptochromes/chemistry
- DNA, Kinetoplast/chemistry
- Deoxyribodipyrimidine Photo-Lyase/chemistry
- Electrophoresis, Polyacrylamide Gel
- Fluorescent Antibody Technique
- Gene Expression Regulation, Enzymologic
- Genome, Protozoan
- Molecular Sequence Data
- Protozoan Proteins/chemistry
- Rabbits
- Trypanosoma cruzi/chemistry