Trypanosoma cruzi: A kinetoplast-associated protein of the photolyase/cryptochrome family

M Carolaing Gabaldón, Luis Labrador, Gloria Arraiz, Juan L. Concepción, Luisana Avilan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A photolyase-like protein gene found in the Trypanosoma cruzi genome database was cloned and expressed in Escherichia coli resulting in the formation of inclusion bodies. Antibodies against this protein were used to determine expression of the protein in the different forms of the parasite. It was visualized in the epimastigote form but not in amastigote or trypomastigote forms obtained from culture in Vero cells. In epimastigotes, this protein is located at the level of the mitochondrion associated to both sides of the kinetoplast. Sequence analyses indicated that this protein, as well as other photolyases from Leishmania spp. and Trypanosoma brucei are related to single-stranded photolyases or cryptochromes DASH.

Original languageEnglish
Pages (from-to)350-356
Number of pages7
JournalExperimental Parasitology
Volume124
Issue number3
Early online date26 Nov 2009
DOIs
Publication statusPublished - Mar 2010

Keywords

  • Amino Acid Sequence
  • Animals
  • Antibodies, Protozoan
  • Blotting, Western
  • Cloning, Molecular
  • Cryptochromes/chemistry
  • DNA, Kinetoplast/chemistry
  • Deoxyribodipyrimidine Photo-Lyase/chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Enzymologic
  • Genome, Protozoan
  • Molecular Sequence Data
  • Protozoan Proteins/chemistry
  • Rabbits
  • Trypanosoma cruzi/chemistry

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